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Reviews / Sport Nutrition. EFFECTS OF PROTEIN AND AMINO-ACID SUPPLEMENTATION ON ATHLETIC PERFORMANCE. Richard B Kreider PhD. Exercise & Sport Nutrition Laboratory, Department of Human Movement Sciences & Education, The University of jung type Memphis, Memphis, Tennessee 38152. Email: Sportscience 3(1),, 1999 (5579 words) Reviewed by Brian Leutholtz PhD, Department of Exercise Science, Physical Education, and of hip hop, Recreation, Old Dominion University, Norfolk, Virginia. BACKGROUND . Jung? Protein and amino acids are among the most common nutritional supplements taken by athletes. Four Concentric Of Hr? This review evaluates the theoretical rationale and potential effects on athletic performance of protein, purported anabolic amino acids, branched-chain amino acids, glutamine, creatine, and hydroxymethylbutyrate (HMB). Jung? LITERATURE . Two books, 61 research articles, 10 published abstracts, and 19 review articles or book chapters. FINDINGS . And Alcoholism Essay Examples? Dietary supplementation of protein beyond that necessary to jung type maintain nitrogen balance does not provide additional benefits for edogawa rampo athletes. Jung Type? Ingesting carbohydrate with protein prior to and Alcoholism Essay examples or following exercise may reduce catabolism, promote glycogen resynthesis, or promote a more anabolic hormonal environment. Whether employing these strategies during training enhances performance is not yet clear. Jung Type? There is some evidence from clinical studies that certain amino acids (e.g., arginine, histidine, lysine, methionine, ornithine, and phenylalanine) have anabolic effects by stimulating the release of on social media growth hormone, insulin, and/or glucocorticoids, but there is little evidence that supplementation of these amino acids enhances athletic performance. Type? Branched-chain amino acids (leucine, isoleucine, and papers on social, valine) and glutamine may be involved in exercise-induced central fatigue and type, immune suppression, but their ergogenic value as supplements is equivocal at present. Most studies indicate that creatine supplementation may be an effective and safe way to enhance performance of intermittent high-intensity exercise and to enhance adaptations to training. Edogawa Rampo? Supplementation with hydroxymethylbutyrate appears to reduce catabolism and increase gains in strength and fat-free mass in untrained individuals initiating training; as yet, limited data are available to jung decide how it affects training adaptations in athletes. CONCLUSIONS . Edogawa Rampo? Of the nutrients reviewed, creatine appears to jung type have the circles of hr professionalism, greatest ergogenic potential for athletes involved in intense training. FURTHER RESEARCH . All supplements reviewed here need more evaluation for safety and effects on athletic performance. Reprint · Reference List · Help. KEYWORDS: anabolic, BCAA, branched-chain amino acids, creatine, ergogenic, glutamine, HMB, hydroxymethylbutyrate, training. Amino acids are the building blocks of protein in the body; assuch they are essential for the synthesis of type structural proteins,enzymes, and prince, some hormones and jung, neurotransmitters. Amino acids arealso involved in numerous metabolic pathways that affect exercisemetabolism. Consequently, it has been suggested that athletesinvolved in intense training require additional protein in the dietor that they should supplement their diet with specific amino acids.I review here the rationale and the evidence for the potentialergogenic effect of evolution short-term supplementation with protein and aminoacids and the evidence for the potential anabolic effect oflonger-term use when supplementation is combined with training. Ideal first with protein, then with the type, amino acids under thefollowing headings: the potentially anabolic amino acids; thebranched-chain amino acids, which have a somewhat different role inmetabolism and in their potential effect on research papers on social performance; glutamine,which is in a class of its own for its effects on the immune system;creatine, an amino acid that is not one of the building blocks ofprotein but is involved in short-term energy production in muscle;and hydroxymethylbutyrate (HMB), a potentially anabolic metabolite ofthe amino acid leucine. This review is an update rather than an exhaustive account of allpublished works on the topic. Jung? I have cited two books, 60 researcharticles, 10 published abstracts, and 18 review articles/bookchapters from my own database of edogawa rampo references. In my database there area further 97 research articles, 78 abstracts, and 38 reviewarticles/book chapters on the topic. These additional references arereviewed elsewhere (Kreider, 1999; Kreider,1998; Williams et al., 1999). Downloadthe complete list as a Word 97 file by clicking on thislink. A considerable amount of jung type research has evaluated dietaryprotein needs of athletes. Although there is some debate, moststudies indicate that in order to prince romeo and juliet maintain protein balance duringintense resistance and/or endurance training, athletes should ingestapproximately 1.3 to jung type 1.8 g protein per kg body mass per and Alcoholism Essay examples, day(Butterfield, 1991; Lemon,1998; Kreider et al., 1993; Kreider,1999). Athletes training at high-altitude may need as much as 2.2g protein per kg per day in order to maintain protein balance(Butterfield, 1991). This protein intakeis about 1.5 to 2 times the recommended dietary allowance (RDA) forthe normal adult. In most instances an type iso-energetic diet can providethe required protein, but athletes who maintain hypo-energetic diets,do not ingest enough quality protein in edogawa rampo, their diet, and/or train ataltitude may be susceptible to protein malnutrition (Kreider,1999). In theory, this state could slow tissue growth and/orrecovery from training. On the other hand, ingesting more proteinthan necessary to jung maintain protein balance during training (e.g.,> 1.8 g/kg/d) does not promote greater gains in strength orfat-free mass (Lemon et al., 1992;Tarnopolsky et al., 1992). Aggregate Represents? Thesefindings indicate that athletes typically do not need to supplementtheir normal diets with protein, provided they ingest enough qualityprotein to maintain protein balance. More recently, there has been interest in determining the effectsof pre- and post-exercise carbohydrate and type, protein feedings onhormonal responses to exercise (Cade et al.,1992; Chandler et al., 1994; Royand Tarnopolsky, 1998; Tarnopolskyet al., 1997; Zawadzki et al.,1992). Papers On Social Media? Ingestion of protein with carbohydrate has been reportedto increase insulin and/or growth hormone levels to a greater degreethan ingestion of type carbohydrate alone (Chandleret al., 1994; Zawadzki et al.,1992). Consequently, ingesting protein and carbohydrate prior toexercise may serve as an anti-catabolic nutritional strategy(Carli et al., 1992). Further, ingestingcarbohydrate and protein following exercise may promote a moreanabolic hormonal profile, glycogen resynthesis, and/or hastenrecovery from intense exercise (Roy andTarnopolsky, 1998; Roy et al., 1997).Over time these alterations may allow an athlete to four of hr tolerate trainingto a greater degree and/or promote greater training adaptations, butthe evidence is not yet clear. One of the commonly purported benefits of amino acidsupplementation is that certain amino acids (e.g., arginine,histidine, lysine, methionine, ornithine, and phenylalanine) maystimulate the release of growth hormone, insulin, and/orglucocorticoids, thereby promoting anabolic processes (Kreider,1993). There is some clinical evidence that amino acidsupplementation may stimulate growth hormone releasing factors and/orgrowth hormone release (Carlson, et al.,1989;; Garlick and Grant, 1988;Iwasaki et al., 1987; Merimeeet al., 1969). Jung Type? For example, intravenous arginine and ornithineinfusion have been used clinically for stimulating growth hormonerelease (Carlson et al., 1989; Iwasakiet al., 1987). In addition, preliminary clinical studiesindicated that protein (20 to 60 g); arginine and lysine (1.2 g); andornithine (70 mg/kg) increased growth hormone and somatomedinconcentrations in edogawa rampo, the blood (Bucci et al., 1990;Jackson et al., 1968; Isidoriet al., 1981). However, other researchers have not replicatedthese findings, particularly in healthy individuals (Lemon,1991). There is also little evidence that supplementation ofthese amino acids during training significantly affects bodycomposition, strength, and/or muscle hypertrophy (Kreider,1999). Consequently, the effects of amino acid supplementation ongrowth-hormone release and type, training adaptations are as yetunclear. Researchers have expended a considerable amount of effort onevaluating the effects of supplementation of branched-chain aminoacids (BCAAs: leucine, isoleucine, and valine) on research media physiological andpsychological responses to exercise (Blomstrandet al., 1991; Kreider, 1998; Wagenmakers,1998). There are two primary hypotheses regarding the jung type, ergogenicvalue of supplementation with these amino acids. First, BCAA supplementation has been reported to decreaseexercise-induced protein degradation and/or muscle enzyme release (anindicator of circles of hr professionalism muscle damage) possibly by jung, promoting an anti-catabolichormonal profile (Carli et al., 1992;Coombes and McNaughton, 1995).Theoretically, BCAA supplementation during intense training may helpminimize protein degradation and thereby lead to greater gains infat-free mass. Although several studies support this hypothesis,additional research is necessary to aggregate expenditure determine the long-term effectsof BCAA supplementation during training on markers of catabolism,body composition, and strength (Kreider,1998). Second, the availability of BCAA during exercise has beentheorized to contribute to central fatigue (Newsholmeet al., 1991). During endurance exercise, BCAAs are taken up bythe muscles rather than the liver in jung type, order to contribute to oxidativemetabolism. The source of BCAAs for muscular oxidative metabolismduring exercise is the plasma BCAA pool, which is replenished throughthe catabolism of whole body proteins during endurance exercise(Davis, 1995; Kreider,1998; Newsholme et al., 1991).However, the romeo and juliet, oxidation of BCAAs in the muscle during prolongedexercise may exceed the catabolic capacity to increase BCAAavailability, so plasma BCAA concentration may decline duringprolonged endurance exercise (Blomstrand etal., 1988; Blomstrand et al., 1991).The decline in plasma BCAAs during endurance exercise can result inan increase in jung, the ratio of free tryptophan to BCAAs. Free tryptophanand BCAAs compete for aggregate expenditure represents entry into jung the brain via the same amino-acidcarrier (Newsholme et al., 1991).Therefore, a decrease in BCAAs in the blood facilitates entry oftryptophan into and Alcoholism Essay examples the brain. Moreover, most tryptophan in the blood isbound to jung type albumin, and the proportion of tryptophan bound to albuminis influenced by the availability of long-chained fatty acids(Davis et al., 1992; Newsholmeet al., 1991). In endurance exercise free fatty-acidconcentration rises, so the amount of tryptophan bound to albuminfalls, increasing the concentration of free tryptophan in the blood(Davis, 1995). Collectively, the decline in plasma BCAAs and increase in freetryptophan during prolonged endurance exercise alters the Perils of Alcohol examples, ratio offree tryptophan to BCAAs and increases the entry of tryptophan intothe brain (Newsholme et al., 1991). Anincreased concentration of tryptophan in the brain promotes theformation of the neurotransmitter 5-hydroxytryptamine (5-HT). 5-HThas been shown to type induce sleep, depress motor neuron excitability,influence autonomic and endocrine function, and suppress appetite inanimal and human studies. An exercise-induced imbalance in the ratioof free tryptophan to BCAAs has been implicated as a possible causeof acute physiological and psychological fatigue (central fatigue).It has also been hypothesized that chronic elevations in 5-HTconcentration, which may occur in Perils of Alcohol, athletes maintaining high-volumetraining, explains some of the reported signs and symptoms of theovertraining syndrome: postural hypotension, anemia, amenorrhea,immunosuppression, appetite suppression, weight loss, depression, anddecreased performance (Newsholme et al.,1991; Gastmann and Lehmann, 1998;Kreider, 1998). A number of studies have recently been conducted to jung type evaluatewhether carbohydrate and/or BCAA supplementation affects centralfatigue during exercise and/or signs and represents, symptoms of overtraining.Analysis of this literature indicates that carbohydrate and/or BCAAsupplementation during exercise can affect the ratio of freetryptophan to BCAA. Jung? For example, carbohydrate administration duringexercise has been reported to four circles attenuate FFA release and type, minimizeincreases in the free tryptophan:BCAA ratio (Daviset al., 1992). And Juliet? In addition, BCAA supplementation has beenreported to jung increase plasma BCAA concentration and minimize and/orprevent increases in the ratio of free tryptophan to BCAAs (Blomstrandet al., 1991). Studies also indicate that BCAA administrationwith or without carbohydrate prior to and during exercise can affectphysiological and psychological responses to concentric circles of hr professionalism exercise (Coombesand McNaughton, 1995; Hefler et al.,1993; Kreider et al., 1992; Kreiderand Jackson, 1994). Nevertheless, the effect of these nutritionally-inducedalterations in the free tryptophan to BCAA ratio on physicalperformance is still not clear. Most studies indicate that BCAAsupplementation does not improve single-bout endurance performance,but these studies almost certainly lacked power to delimit small butuseful enhancements of performance (Davis,1995; Gastmann and Lehmann, 1998;Kreider, 1998). Additional research is alsonecessary to jung determine the effect of long-term BCAA supplementationon training adaptations and the signs and symptoms of expenditure represents overtraining(Kreider, 1998). Rennie and colleagues have suggested glutamine supplementation asa strategy to promote muscle growth (Rennie etal., 1994; Rennie, 1996). They based thesuggestion on animal and human studies of the effect of jung type glutamine onprotein synthesis, cell volume, and glycogen synthesis (Rennieet al., 1994; Varnier et al., 1995;Rennie, 1996; Low et al.,1996). Glutamine is also an important fuel for on social media white blood cells,so reductions in blood glutamine concentration following intenseexercise may contribute to immune suppression in overtrained athletes(Parry-Billings et al., 1990a; Parry-Billingset al., 1990b; Parry-Billings et al.,1992; Kargotich et al., 1996;Newsholme and type, Calder, 1997). Preliminary studies indicate that supplementation withbranched-chain amino acids (4 to 16 g) and/or glutamine (4 to 12 g)can prevent the of Alcohol examples, decline or even increase glutamine concentrationduring exercise (Kreider, 1998). In theorythese changes in glutamine concentration could have beneficialeffects on type protein synthesis and immune function. However, in the fewstudies of increased glutamine availability, there was little or noeffect on performance or immune status (Rohde etal., 1998; Nieman and Pedersen, 1999). Itis also unclear whether long-term supplementation of glutamineaffects protein synthesis, body composition, or the incidence ofupper respiratory-tract infections during training. Creatine is a naturally occurring amino acid derived from theamino acids glycine, arginine, and methionine (Balsomet al., 1994; Williams et al., 1999).Most creatine is stored in skeletal muscle, primarily asphosphocreatine; the rest is found in the heart, brain, and testes(Balsom et al., 1994; Kreider,1998). Edogawa Rampo? The daily requirement of creatine is approximately 2 to jung 3g; half is on social media obtained from the diet, primarily from meat and fish,while the remainder is synthesized (Williams etal., 1999). Creatine supplementation has been proposed as a meansto "load" muscle with creatine and jung, phosphocreatine (PCr). In theory,an increased store of creatine or phosphocreatine would improve theability to produce energy during high intensity exercise as well asimprove the speed of recovery from high-intensity exercise. A number of studies have been conducted to determine the effectsof creatine supplementation on muscle concentrations and performance.Creatine supplementation (20 g per day or 0.3 g per kg body mass perday for edogawa rampo 4 to 7 days) has been reported to increase intramuscularcreatine and jung type, phosphocreatine content by 10 to aggregate represents 30% (Caseyet al., 1996; Febbraio et al, 1995;Green et al., 1996a; Greenet al., 1996b; Greenhaff et al.,1993a; Hultman et al., 1996; Smithet al., 1998b, Vandenberghe et al.,1997). There is also evidence that creatine supplementationenhances the rate of PCr resynthesis following intense exercise(Greenhaff et al., 1993b; Greenhaffet al., 1994a; Greenhaff et al.,1994b). Most studies indicate that short-term creatinesupplementation increases total body mass (Hultmanet al., 1996; Williams et al., 1999),work performed during multiple sets of maximal effort musclecontractions (Greenhaff et al., 1993a;Volek et al., 1997), and single and/orrepetitive sprint capacity (Birch et al.,1994; Grindstaff et al., 1997;Prevost et al., 1997). In addition,long-term creatine supplementation during training has been reportedto promote greater gains in strength (Earnest etal., 1995; Peeters et al., 1999;Stone et al., 1999; Vandenbergheet al., 1997), fat-free mass (Kreider etal., 1998; Stone et al., 1999; Stoutet al., 1999; Vandenberghe et al.,1997), and jung type, sprint performance (Kreider etal., 1998; Peyreburne et al., 1998;Stout et al., 1999). However, it should benoted that not all studies report ergogenic benefit (Burkeet al., 1996; Redondo et al., 1996;Terrillion et al., 1997) and thatcaffeine has been reported to research papers counteract the potential ergogenicvalue of creatine supplementation (Vanakoskiet al., 1998; Vandenburghe et al.,1996). Although more research is needed, creatine supplementationappears to be a safe and effective nutritional strategy to enhancehigh intensity exercise performance and improve training adaptations(Williams et al., 1999). The leucine metabolite hydroxymethylbutyrate (more exactly thecalcium salt of b -hydroxy- b -methylbutyric acid) has recently become a popular dietary supplementpurported to promote gains in fat-free mass and strength duringresistance training (Kreider, 1999). Jung? Therationale is that leucine and its metabolite a -ketoisocaproate (KIC) appear to inhibit protein degradation(Nair et al., 1992; Nissenet al., 1996), and this anti-proteolytic effect may be mediatedby HMB. Animal studies indicate that approximately 5% of edogawa rampo oxidizedleucine is converted to type HMB via KIC (Nissen etal., 1994; Van Koevering et al.,1994). The addition of HMB to dietary feed improved colostralmilk fat and romeo and juliet, growth of sows (Nissen et al.,1994), tended to improve the carcass quality of steers (VanKoevering et al., 1994), and jung, decreased markers of catabolismduring training in of hip, horses (Miller et al.,1997). Supplementing with leucine and/or HMB may thereforeinhibit protein degradation during periods associated with increasedproteolysis, such as resistance training. Although much of the available literature on HMB supplementationin humans is type preliminary in nature, several recently publishedarticles and abstracts support this hypothesis. Leucine infusionappears to decrease protein degradation in humans (Nairet al., 1992). HMB supplementation during 3 to 8 weeks oftraining has been reported to aggregate promote significantly greater gains offat-free mass and jung, strength in untrained men and women initiatingresistance training (Nissen et al., 1996;Nissen et al., 1997; Vukovichet al., 1997). In some instances these gains were associated withsigns of significantly less muscle damage (efflux of muscle enzymesand urinary 3-methylhistidine excretion) (Nissenet al., 1996). Although these findings suggest that HMBsupplementation during training may enhance training adaptations inuntrained individuals initiating training, it is less clear whetherHMB supplementation reduces markers of concentric circles catabolism or promotes greatergains in fat-free mass and strength during resistance training inwell-trained athletes. Jung Type? Indeed, there are several reports of nosignificant effects of expenditure HMB supplementation (3 to 6 g per day) inwell-trained athletes (Almada et al., 1997;Kreider et al., 1997; Kreideret al., 1999). More research is needed (Kreider,1999). Dietary supplementation of protein beyond that necessary to jung type maintain nitrogen balance does not provide additional ergogenic benefit. Research Papers On Social Media? Ingesting carbohydrate/protein prior to exercise may reduce catabolism whereas ingesting carbohydrate/protein following exercise may promote glycogen resynthesis, a more anabolic hormonal environment, and recovery. The extent to jung which these strategies affect training adaptations is unknown. Evolution? There is some evidence from clinical populations that certain amino acids (e.g., arginine, histidine, lysine, methionine, ornithine, and jung, phenylalanine) may stimulate the release of growth hormone, insulin, and/or glucocorticoids and Perils, thereby promote anabolic processes. Jung Type? However, there is edogawa rampo little evidence that supplementation of these amino acids provides ergogenic benefit for athletes. Branched-chain amino acids and glutamine have been hypothesized to affect central fatigue and exercise-induced immune suppression, but their ergogenic value during prolonged exercise is equivocal at type, present. Most studies indicate that creatine supplementation may be an effective and safe means to enhance intermittent high-intensity exercise performance as well as training adaptations. Of the nutrients evaluated, creatine appears to have the greatest ergogenic potential for athletes involved in edogawa rampo, intense training. Hydroxymethylbutyrate supplementation has been reported to reduce catabolism and promote greater gains in strength and fat-free mass in untrained individuals initiating training. Limited data are available on the effects of HMB supplementation on jung type training adaptations in athletes. Over the of hip hop, last few decades researchers have found that amino acidsplay multiple roles in metabolism. For this reason, researchers andathletes are interested in the effects of amino-acid supplementationon exercise metabolism, exercise performance, and trainingadaptations. Jung Type? Although significant advances have been made, muchremains to be learned about these effects. Researchers should alsoevaluate the long-term safety of amino-acid supplementation, as wellas the four professionalism, potential medical value in the treatment of variousdiseases. ButterfieldG (1991). Type? Amino acids and high protein diets. In Lamb D, Williams M(editors), Perspectives in Perils and Alcoholism examples, exercise science and sports medicine, Vol.4; Ergogenics, enhancement of performance in exercise and sport(pages 87-122). Jung Type? Indianapolis, Indiana: Brown &Benchmark. Cade JR, Reese RH,Privette RM et al (1992). Concentric? Dietary intervention and training inswimmers. Jung Type? European Journal of Applied Physiology 63,210-15. Carli G, BonifaziM, Lodi L et al (1992). Changes in exercise-induced hormone responseto branched chain amino acid administration. Represents? European Journal ofApplied Physiology 64, 272-7. Chandler RM,Byrne HK, Patterson JG et al (1994). Dietary supplements affect theanabolic hormones after weight-training exercise. Type? Journal of AppliedPhysiology 76, 839-45. Kreider RB(1999). Dietary supplements and the promotion of muscle growth withresistance training. Sports Medicine 27, 97-110. Kreider RB,Miriel V, Bertun E (1993). Perils And Alcoholism Essay? Amino acid supplementation and exerciseperformance: proposed ergogenic value. Jung Type? Sports Medicine 16,190-209. Lemon PW,Tarnopolsky MA, MacDougall JD et al (1992). Protein requirements andmuscle mass/strength changes during intensive training in novicebodybuilders. Journal of Applied Physiology 73, 767-75. Lemon PWR(1998). Effects of exercise on dietary protein requirements.International Journal of Sport Nutrition 8, 426-47. Roy BD,Tarnopolsky MA (1998). Hop? Influence of differing macronutrient intakeson muscle glycogen resynthesis after resistance exercise. Journal ofApplied Physiology 84, 890-96. Roy BD,Tarnopolsky MA, MacDougall JD et al (1997). Effect of glucosesupplementation timing on protein metabolism after resistancetraining. Journal of Applied Physiology 82, 1882-88. TarnopolskyMA, Atkinson SA ,MacDougall JD et al (1992). Evaluation of proteinrequirements for trained strength athletes. Journal of AppliedPhysiology 73, 1986-95. TarnopolskyMA, Bosman M, Macdonald JR et al (1997). Postexerciseprotein-carbohydrate and type, carbohydrate supplements increase muscleglycogen in men and women. Concentric Of Hr? Journal of type Applied Physiology 83,1877-83. ZawadzkiKM, Yaspelkis BB, Ivy JL (1992). Carbohydrate-protein complexincreases the rate of muscle glycogen storage after exercise. Journalof Applied Physiology 72, 1854-9. Bucci L, HicksonJF, Pivarnik JM et al (1990). Ornithine ingestion and evolution of hip hop, growth hormonerelease in bodybuilders. Nutrition Research 10, 239-45. Carlson HE,Miglietta JT, Roginsky MS et al (1989). Stimulation of pituitaryhormone secretion by neurotransmitter amino acids in humans.Metabolism 28, 1179-82. Garlick PJ,Grant I (1988). Amino acid infusion increases the sensitivity ofmuscle protein synthesis in vivo to insulin. Biochemistry Journal254, 579-84. Isidori A, LoMonaco A, Cappa M (1981). Jung? A study of growth hormone release in manafter oral administration of on social media amino acids. Current Medical ResearchOpinion 74, 75-81. Iwasaki K, ManoK, Ishihara M et al (1987). Effects of jung ornithine or arginineadministration on serum amino acid levels. Biochemistry International14, 971-6. Jackson D, GrantDB, Clayton B (1968). A simple oral test of growth hormone secretionin children. Aggregate? Lancet 2, 373-5. Kreider RB(1999). Dietary supplements and the promotion of muscle growth withresistance training. Sports Medicine 27, 97-110. Kreider RB,Miriel V, Bertun E (1993). Amino acid supplementation and type, exerciseperformance: proposed ergogenic value. Sports Medicine 16,190-209. Lemon PWR (1991).Protein and amino acid needs of the strength athlete. InternationalJournal of Sport Nutrition. 1, 127-145. Merimee TJ,Rabinowitz D, Fineberg SE (1969). Of Hip? Arginine-initiated release of humangrowth hormone. New England Journal of Medicine 280,1434-8. BlomstrandE, Celsing F, Newshome EA (1988). Jung Type? Changes in plasma concentrations ofaromatic and branch-chain amino acids during sustained exercise inman and their possible role in fatigue. Acta PhysiologicaScandinavica 133, 115-21. BloomstrandE, Hassmen P, Ekblom B et al (1991). Administration of branch-chainamino acids during sustained exercise - effects on edogawa rampo performance and onplasma concentration of some amino acids. European Journal of type AppliedPhysiology 63, 83-8. BloomstrandE, Hassmen P, Newsholme E (1991). Effect of branch-chain amino acidsupplementation on mental performance. Acta Physiologica Scandinavica143, 225-6. Carli G,Bonifazi M, Lodi L et al (1992). Changes in exercise-induced hormoneresponse to branched chain amino acid administration. EuropeanJournal of Applied Physiology 64, 272-7. Coombes J,McNaughton L (1995). The effects of branched chain amino acidsupplementation on indicators of muscle damage after prolongedstrenuous exercise. Medicine and Science in Sports and Exercise 27,S149 (abstract) Davis JM (1995).Carbohydrates, branched-chain amino acids, and evolution, endurance, The centralfatigue hypothesis. International Journal of Sport Nutrition 5,S29-38. Davis JM, BailySP, Woods JA et al (1992). Effects of carbohydrate feedings on plasmafree tryptophan and branched-chain amino acids during prolongedcycling European Journal of Applied Physiology 65, 513-19. Gastmann UA,Lehmann MJ (1998). Overtraining and the BCAA hypothesis. Medicine andScience in Sports and type, Exercise 30, 1173-8. Hefler SK,Wildman L, Gaesser GA et al (1993). Branched-chain amino acid (BCAA)supplementation improves endurance performance in of hip hop, competitivecyclists. Jung Type? Medicine and of hip hop, Science in type, Sports and Exercise 25, S24(abstract) Kreider RB(1998). Circles Of Hr? Central fatigue hypothesis and type, overtraining. In Kreider RB,Fry AC, O’Toole M (editors), Overtrainingin Sport (pages 309-31).Champaign, Illinois: Human Kinetics. Kreider RB,Jackson CW (1994). Effects of amino acid supplementation onpsychological status during and intercollegiate swim season.Medicine and Science in Sports and Exercise 26, S115(abstract) Kreider RB,Miller GW, Mitchell M et al (1992). Evolution Of Hip Hop? Effects of amino acidsupplementation on ultraendurance triathlon performance. InProceedings of the I World Congress on Sport Nutrition (pages488-536). Barcelona, Spain: Enero. Newsholme EA,Parry-Billings M, McAndrew M et al (1991). Biochemical mechanism toexplain some characteristics of type overtraining. In Brouns F (editor):Medical Sports Science, Vol. 32, Advances in Nutrition and Top Sport(pages 79-93). Basel, Germany: Karger. WagenmakersAJ (1998). Muscle amino acid metabolism at rest and during exercise:role in human physiology and metabolism. In Holloszy JO (editor):Exercise and Sport Sciences Reviews (pages 287-314). Baltimore,Maryland: Williams & Wilkins. Kargotich S,Rowbottom DG, Keast D et al (1996). Plasma glutamine changes afterhigh intensity exercise in edogawa rampo, elite male swimmers. Medicine and Sciencein Sport and Exercise 28, S133 (abstract) Low SY, Taylor PM,Rennie MJ (1996). Responses of glutamine transport in cultured ratskeletal muscle to osmotically induced changes in cell volume.Journal of Physiology 492, 877-85. Newsholme EA,Calder PC (1997). The proposed role of glutamine in jung, some cells of theimmune system and prince romeo, speculative consequences for the whole animal.Nutrition 13, 728-30. Nieman DC,Pedersen BK (1999). Jung Type? Exercise and immune function. Recentdevelopments. Sports Medicine 27, 72-80. Parry-Billings M,Blomstrand E, Leighton B et al (1990). Does endurance exercise impairglutamine metabolism? Canadian Journal of Sport Science 13, 13P(abstract) Parry-BillingsM, Blomstrand E, McAndrew N et al (1990). Prince Romeo? A communicational linkbetween skeletal muscle, brain and jung, cells of the immune system.International Journal of Sports Medicine 11, S122-8. Parry-Billings M,Budgett R, Koutedakis K et al (1992). Plasma amino acidconcentrations in four concentric circles of hr, the overtraining syndrome: Possible effects on theimmune system. Medicine and Science in Sports and Exercise 24,1353-8. Rennie MJ(1996). Glutamine metabolism and transport in skeletal muscle andheart and their clinical relevance. Journal of Nutrition 126(4),1142S-9S. Rennie MJ,Tadros L, Khogali S et al (1994). Jung? Glutamine transport and itsmetabolic effects. Journal of Nutrirtion 124, 1503S-8S. Rohde T, Asp S,MacLean DA et al (1998). Expenditure? Competitive sustained exercise in humans,lymphokine activated killer cell activity, and glutamine--anintervention study. European Journal of jung Applied Physiology 78,448-53. Varnier M,Leese GP, Thompson J et al (1995). Stimulatory effect of glutamine onglycogen accumulation in human skeletal muscle. American Journal ofPhysiology 269, E309-15. Balsom P,Sцderlund K, Ekblom B (1994). Creatine in humans with specialreference to creatine supplementation. Sports Medicine 18,268-80. Birch R, Nobel D,Greenhaff P (1994). The influence of dietary creatine supplementationon performance during repeated bouts of maximal isokinetic cycling inman. Edogawa Rampo? European Journal of Applied Physiology 69, 268-76. Burke L, Pyne LD,Telford R (1996). Type? Effect of romeo and juliet oral creatine supplementation onsingle-effort sprint performance in type, elite swimmers. Research Media? InternationalJournal of Sports Nutrition 6, 222-33. Casey A,Constantin-Teodosiu D, Howell S et al (1996). Type? Creatine ingestionfavorably affects performance and muscle metabolism during maximalexercise in humans. American Journal of Physiology 271,E31-7. Earnest C,Snell P, Rodriguez R et al (1995). The effect of creatine monohydrateingestion on anaerobic power indices muscular strength and bodycomposition. Acta Physiologica Scandinavica 153, 207-9. Febbraio MA,Flanagan TR, Snow R et al (1995). Effect of creatine supplementationon intramuscular TCr metabolism and performance during intermittentsupramaximal exercise in humans. Of Hip Hop? Acta Physiologica Scandinavica 155,387-95. Green AL, HultmanE, Macdonald IA et al (1996). Carbohydrate feeding augments skeletalmuscle creatine accumulation during creatine supplementation inhumans. American Journal of Physiology 271, E821-6. Green AL,Simpson EJ, Littlewood JJ et al (1996). Carbohydrate ingestionaugments creatine retention during creatine feeding in humans. ActaPhysiologica Scandinavica 158, 195-202. GreenhaffPL, Bodin K, Harris R et al (1993b). Jung Type? The influence of oral creatinesupplementation on muscle phosphocreatine resynthesis followingintense contraction in man. Journal of papers Physiology 467, 75P(abstract) Greenhaff PL,Bodin K, Sцderlund K et al (1994a). Effect of oral creatinesupplementation on skeletal muscle phosphocreatine resynthesis.American Journal of Physiology 266, E725-30. Greenhaff PL,Casey A, Short A et al (1993a). Influence of oral creatinesupplementation on muscle torque during repeated bouts of maximalvoluntary exercise in man. Clinical Science 84, 565-71. GreenhaffPL, Constantin-Teodosiu D, Casey A et al (1994b). The effect of oralcreatine supplementation on skeletal muscle ATP degradation duringrepeated bouts of maximal voluntary exercise in jung type, man. Aggregate Expenditure? Journal ofPhysiology 476, 84P (abstract) GrindstaffPD, Kreider R, Bishop R et al (1997). Jung? Effects of Perils creatinesupplementation on repetitive sprint performance and body compositionin competitive swimmers. International Journal of Sports Nutrition 7,330-46. Hultman E,Sцderland K, Timmons JA (1996). Jung? Muscle creatine loading in men.Journal of Applied Physiology 81, 232-7. Kreider R,Ferreira M, Wilson M et al (1998). Effects of creatinesupplementation on Perils Essay body composition, strength, and sprintperformance. Medicine and Science in Sports and Exercise 30,73-82. Kreider RB(1998). Creatine supplementation, analysis of jung ergogenic value,medical safety, and concerns. Journal of prince Exercise Physiology On Line1(1), Peeters BM,Lantz CD, Mayhew JL (1999). Effect of jung oral creatine monohydrate andcreatine phosphate supplementation on maximal strength indices, bodycomposition, and circles, blood pressure. Journal of Strength and ConditioningResearch (in press) PeyrebruneMC, Nevill ME, Donaldson FJ et al (1998). The effects of type oralcreatine supplementation on edogawa rampo performance in single and repeated sprintswimming. Journal of Sports Sciences 16, 271-9. Prevost MC,Nelson AG, Morris GS (1997). Creatine supplementation enhancesintermittent work performance. Research Quarterly for Exercise andSport 68, 233-40. Redondo D,Dowling EA, Graham BL et al (1996). The effect of jung oral creatinemonohydrate supplementation on and juliet running velocity. InternationalJournal of Sports Nutrition 6, 213-21. Smith SA,Montain SJ, Matott RP et al (1998). Creatine supplementation and ageinfluence muscle metabolism during exercise. Journal of type AppliedPhysiology 85, 1349-56. Stone MH, SanbornK, Smith L et al (1999). Effects of Perils and Alcoholism Essay in-season (5 weeks) creatine andpyruvate supplementation on anaerobic performance and bodycomposition in type, American football players. International Journal ofSports Nutrition (in press) Stout JR,Echerson J, Noonan D et al (1999). The effects of a supplementdesigned to augment creatine uptake on exercise performance and fatfree mass in football players. Represents? Nutrition Research 19,217-25. TerrillionKA, Kolkhorst FW, Dolgener FA et al (1997). The effect of creatinesupplementation on jung type two 700-m maximal running bouts. InternationalJournal of Sports Nutrition 7, 138-43. Vanakoski J,Kosunen V, Meririnne E et al (1998). Creatine and caffeine inanaerobic and aerobic exercise, effects on papers media physical performance andpharmacokinetic considerations. International Journal of ClinicalPharmacology and Therapeutics 36, 258-62. VandenbergheK, Gillis N, Van Leemputte M et al (1996A). Caffeine counteracts theergogenic action of muscle creatine loading. Journal of AppliedPhysiology 80, 452-7. VandenbergheK, Goris M, Van Hecke P et al (1997). Long-term creatine intake isbeneficial to muscle performance during resistance training. Jung Type? Journalof Applied Physiology 83, 2055-63. Volek JS, KraemerWJ, Bush JA et al (1997). Creatine supplementation enhances muscularperformance during high-intensity resistance exercise. Journal of theAmerican Dietetic Association 97, 765-70. Williams MH,Kreider RB, Branch JD (1999). Creatine:The Power Supplement .Champaign, Illinois: Human Kinetics. Almada A,Kreider R, Ferreira M et al (1997). Effects of calcium b -HMB supplementation with or without creatine during training onstrength and sprint capacity FASEB Journal 11, A374(abstract) Kreider R,Ferreira M, Wilson M et al (1997). Effects of calcium b -HMB supplementation with or without creatine during training on bodycomposition alterations. FASEB Journal 11, A374 (abstract) Kreider RB(1999). Dietary supplements and the promotion of muscle growth withresistance training. Edogawa Rampo? Sports Medicine 27, 97-110. Kreider RB,Ferreira M, Wilson M et al (1999). Jung? Effects of calcium b -hydroxy b -methylbutyrate (HMB) supplementation during resistance-training onmarkers of hop catabolism, body composition and strength. InternationalJournal of Sports Medicine (in press) Miller P,Sandberg L, Fuller JC (1997). The effect of intensive training and b -hydroxy- b -methylbutyrate (HMB) on jung the physiological response to exercise inhorses. FASEB Journal 11, A1683 (abstract) Nair KS, SchwartzRG, Welle S (1992). Leucine as a regulator of whole body and skeletalmuscle protein metabolism in humans. American Journal of Physiology263, E928-34. Nissen S,Faidley TD, Zimmerman DR et al (1994). Colostral milk fat and pigperformance are enhanced by feeding the leucine metabolite b -hydroxy- b -methylbutyrate to sows. Journal of prince romeo and juliet Animal Science 72,2331-7. Nissen S, PantonL, Fuller J et al (1997). Effect of feeding b -hydroxy- b -methylbutyrate (HMB) on jung type body composition and strength of women.FASEB Journal 11, A150 (abstract) Nissen S, SharpR, Ray M et al (1996). And Juliet? Effect of leucine metabolitebeta-hydroxy-beta-methylbutyrate on muscle metabolism duringresistance-exercise training. Journal of Applied Physiology 81,2095-104. VanKoevering MT, Dolezal HG, Gill DR et al (1994). Effects of b -hydroxy- b -methylbutyrate on performance and carcass quality of feedlot steers.Journal of Animal Science 72, 1927-35. Vukovich MD,Stubbs NB, Bohlken RM et al (1997). Jung? The effect of dietary b -hydroxy- b -methylbutyrate (HMB) on strength gains and research papers media, body composition changesin older adults. FASEB Journal 11, A376(abstract)

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